raybio mouse mapk pathway phosphorylation array Search Results


raybio mouse mapk pathway phosphorylation array  (RayBiotech inc)
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Farnesylthiosalicylate (FTS) inhibits phosphorylation of mitogen‐activated protein kinases (MAPKs) and T‐cell proliferation following T‐cell antigen receptor (TCR) stimulation. Isolated mouse CD4+ T cells were stimulated with anti‐‐CD3/28 antibodies and treated with FTS 10 µM (green) or vehicle (red) for 30 minutes. A , Bar graphs depict the relative phosphorylation level (arbitrary units of intensity per densitometry) of the indicated kinases included in <t>the</t> <t>RayBio</t> Mouse <t>MAPK</t> Pathway Phosphorylation Array, as analyzed per the manufacturer’s protocol. B , Flow cytometry data expressed as overlay histograms depicting phosphorylated (p)‐extracellular signal‐regulated kinase (Erk)1/2, p‐AKT, and p‐p38 levels, determined by phospho‐flow‐cytometry with phospho‐specific antibodies, as detailed in the Methods section. T cells treated with FTS or control media are shown in green and red, respectively, whereas unstained counterpart samples are in grey. C , CD4+ T cells were labeled with carboxyfluorescein succinimidyl ester (CFSE) and activated by plate‐bound anti‐CD3 and soluble anti‐CD28 monoclonol antibodies (mAbs), treated with the indicated concentration of FTS, and cultured for 72 hours. Shown is a representative experiment (CFSE‐dilution flow cytometry histograms) out of two independent experiments, and the bar graphs depict mean ± SD of triplicates. Samples were analyzed for statistical significance by Student's t test (*** P < .001, ** P < .01, and * P < .05).
Raybio Mouse Mapk Pathway Phosphorylation Array, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Farnesylthiosalicylate (FTS) inhibits phosphorylation of mitogen‐activated protein kinases (MAPKs) and T‐cell proliferation following T‐cell antigen receptor (TCR) stimulation. Isolated mouse CD4+ T cells were stimulated with anti‐‐CD3/28 antibodies and treated with FTS 10 µM (green) or vehicle (red) for 30 minutes. A , Bar graphs depict the relative phosphorylation level (arbitrary units of intensity per densitometry) of the indicated kinases included in the RayBio Mouse MAPK Pathway Phosphorylation Array, as analyzed per the manufacturer’s protocol. B , Flow cytometry data expressed as overlay histograms depicting phosphorylated (p)‐extracellular signal‐regulated kinase (Erk)1/2, p‐AKT, and p‐p38 levels, determined by phospho‐flow‐cytometry with phospho‐specific antibodies, as detailed in the Methods section. T cells treated with FTS or control media are shown in green and red, respectively, whereas unstained counterpart samples are in grey. C , CD4+ T cells were labeled with carboxyfluorescein succinimidyl ester (CFSE) and activated by plate‐bound anti‐CD3 and soluble anti‐CD28 monoclonol antibodies (mAbs), treated with the indicated concentration of FTS, and cultured for 72 hours. Shown is a representative experiment (CFSE‐dilution flow cytometry histograms) out of two independent experiments, and the bar graphs depict mean ± SD of triplicates. Samples were analyzed for statistical significance by Student's t test (*** P < .001, ** P < .01, and * P < .05).

Journal: ACR Open Rheumatology

Article Title: Inhibition of Ras GTPases prevents Collagen‐Induced Arthritis by Reducing the Generation of Pathogenic CD4 + T Cells and the Hyposialylation of Autoantibodies

doi: 10.1002/acr2.11169

Figure Lengend Snippet: Farnesylthiosalicylate (FTS) inhibits phosphorylation of mitogen‐activated protein kinases (MAPKs) and T‐cell proliferation following T‐cell antigen receptor (TCR) stimulation. Isolated mouse CD4+ T cells were stimulated with anti‐‐CD3/28 antibodies and treated with FTS 10 µM (green) or vehicle (red) for 30 minutes. A , Bar graphs depict the relative phosphorylation level (arbitrary units of intensity per densitometry) of the indicated kinases included in the RayBio Mouse MAPK Pathway Phosphorylation Array, as analyzed per the manufacturer’s protocol. B , Flow cytometry data expressed as overlay histograms depicting phosphorylated (p)‐extracellular signal‐regulated kinase (Erk)1/2, p‐AKT, and p‐p38 levels, determined by phospho‐flow‐cytometry with phospho‐specific antibodies, as detailed in the Methods section. T cells treated with FTS or control media are shown in green and red, respectively, whereas unstained counterpart samples are in grey. C , CD4+ T cells were labeled with carboxyfluorescein succinimidyl ester (CFSE) and activated by plate‐bound anti‐CD3 and soluble anti‐CD28 monoclonol antibodies (mAbs), treated with the indicated concentration of FTS, and cultured for 72 hours. Shown is a representative experiment (CFSE‐dilution flow cytometry histograms) out of two independent experiments, and the bar graphs depict mean ± SD of triplicates. Samples were analyzed for statistical significance by Student's t test (*** P < .001, ** P < .01, and * P < .05).

Article Snippet: Using the RayBio Mouse MAPK Pathway Phosphorylation Array, we determined the relative phosphorylation level of 17 different kinases, including well‐established Ras effectors (Figure ).

Techniques: Isolation, Flow Cytometry, Labeling, Concentration Assay, Cell Culture